DISCUSSION & SUMMARY

•In this study, 48 subjects (82.76%) were detected as fertile. Among the fertile subjects, 26 subjects (54.16%) did not showed any growth, 6 subjects (12.5%) were detected for Ureaplasma urealyticum , and 16 subjects (33.33%) were detected for other organisms. Fried egg colony was detected in blood agar after 72 hrs (without Co2) in 10 samples. We inoculated the fried egg colony in the A7 agar, and broth in the presence of CO2 (using candle jar technique). The A7 broth turns to yellow within 48 hrs of inoculation. We also detected fried egg colony within 48 hrs in the A7 agar medium. Colonies were found in the Zone of inhibition was detected for azithromycin and doxycyclin.

•Infections of the male genitourinary tract account for up to 15% of cases of male infertility (Pellati D,). Acute and chronic infections and consequent inflammation in the male reproductive system may compromise the sperm cell function and the whole spermatogenetic process, causing qualitative and quantitative sperm alterations (Henkel R, Sanocka-Maciejewska D). Recent studies have shown that the simple presence of bacteria in semen samples may compromise the sperm quality. The bacteria responsible for semen contaminations generally originate from the urinary tract of patients or can be transmitted by the partner via sexual intercourse

SUMMARY

•Semen sample was collected from 58 male subjects (mean age = 33), who were referred to Kanmani Fertilty Clinic, Chennai, India. A consent form was taken from the subjects before the sample collection.  Sterile sample was collected for microbiological investigation. Semen analysis was done based on WHO criteria. All the semen parameters were noted. The semen sample was inoculated in Blood agar, Mac Conkey agar and A7 agar. Blood agar and A7 agar showed discrete fried egg colonies of Ureaplasma urealyticum. A7 agar is the selective media for Ureaplasma urealyticum, still for further confirmation Biochemical analysis is done. Negative results were observed for all the IMViC reaction and positive result was observed in urease test. Antimicrobial sensitivity test reveals Azithromycin and doxycycline has effect on Ureaplasma urealyticum. Ureaplasma urealyticum showed enhanced growth in agar plate with Omega - 3 when compared with the normal A7 agar plate. Further studies are required to determine the mechanism behind the enhanced growth while addition of Omega – 3. In future PCR identification of the Ureaplasma urealyticum will be done.