IMViC REACTIONS

• Ureaplasma urealyticum gives negative results for all the IMViC reaction, whereas gives positive result for Urease test. After incubation of the sample the urease slant turns into pink colour.

+    -

-     -      -      -       -      +

DISCUSSION

•The occurrence of bacteria in sperm samples intended for IVF will affect the outcome of ART procedure. Effective semen processing procedures should therefore be implemented to remove bacteria from semen. Unfortunately, technique failure does occur whereby bacteria can be found in processed sperm preparations. This happens when the semen samples were infected with bacteria. To improve the success of the ART procedure, routine analysis of microbial investigation is performed in the semen samples and subjects were given antibiotic course for a specific period of time.

•In this pilot study, we performed semen analysis and microbiological investigation for 58 male subjects (mean Age = 33), who comes for ART procedure. For overall group, 11 subjects were detected for U urealyticum, 29 subjects were not found to have growth, 18 subjects were found to have other organism. Based on WHO sperm parameters, 2010, out of 58 subjects, 10 subjects (17.24%) were detected for subfertility. Among the subfertile subjects, 3 subjects (30%) did not showed microbial growth, 5 subjects (50%) were detected for Ureaplasma urealyticum, and 2 subjects were detected for other organisms (20%).