Semen Sample Investigation

•Semen analysis was assessed according to the guidelines of WHO, 2010.

•Sperm parameters such as sperm motility, viability, count, morphology, liquification, pH, was analyzed.

Table 2: WHO Parameters, 2010

WHO Parameters	Normal Range
Volume	2 Ml
Liquefication Time	20 minutes
Sperm concentration	20*106
Motility	>30%

MICROBIOLOGICAL INVESTIGATION

üStreak plate technique

üPrinciple for A7 agar

üIMViC Reactions

üIndole Test

üMethyl Red (MR) Test

üVoges Proskauer (VP) Test

üCitrate Utilization Test

üUrease test

üAntimicrobial sensitivity